5WWQ

Crystal structure of human NSun6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-04-25
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97852
Spacegroup name	P 21 21 21
Unit cell lengths	66.965, 71.877, 219.030
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.406 - 2.815
R-factor	0.2303
Rwork	0.229
R-free	0.26670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1j2b 2yxl
RMSD bond length	0.002
RMSD bond angle	0.656
Data reduction software	SCALEPACK
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.800
Number of reflections	26419
<I/σ(I)>	15.9
Completeness [%]	97.6
Redundancy	24.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8.6	289	2% Dioxane, 0.1M Bicine, 18% (w/v) PEG 8000, 10 mM Yttrium(III) chloride hexahydrate