5WWO

Crystal structure of Enp1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2011-02-24
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97915
Spacegroup name	P 21 21 21
Unit cell lengths	69.220, 98.607, 109.728
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.751 - 2.400
R-factor	0.1704
Rwork	0.167
R-free	0.22970
Structure solution method	SIRAS
RMSD bond length	0.009
RMSD bond angle	1.111
Data scaling software	SCALEPACK
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.440
High resolution limit [Å]	2.400	6.510	2.400
Rmerge	0.111	0.045	0.577
Total number of observations	205522
Number of reflections	29903
<I/σ(I)>	11.4
Completeness [%]	99.7	95.5	100
Redundancy	6.9	6.3	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	7.2% PEG3350, 12% (v/v) Tacsimate pH 6.0, 8% (v/v) Tacsimate pH 8.0