5WWM
Crystal structure of the TPR domain of Rrp5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-07-07 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 114.526, 114.526, 70.933 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.999 - 2.809 |
| R-factor | 0.2071 |
| Rwork | 0.205 |
| R-free | 0.25800 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.993 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 20.000 | 20.000 | 2.850 |
| High resolution limit [Å] | 2.800 | 7.470 | 2.800 |
| Rmerge | 0.125 | 0.060 | 0.626 |
| Number of reflections | 13319 | ||
| <I/σ(I)> | 11 | ||
| Completeness [%] | 100.0 | 99.4 | 100 |
| Redundancy | 13.6 | 11.9 | 14 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.5M ammonium sulfate, 1.0M lithium sulfate, 0.1M sodium citrate, pH 5.6 |
