5WWL
Crystal structure of the Schizogenesis pombe kinetochore Mis12C subcomplex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-10-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97911 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 41.500, 85.610, 123.790 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.010 - 2.400 |
| R-factor | 0.23596 |
| Rwork | 0.234 |
| R-free | 0.27238 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.776 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AutoSol |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.010 | 2.550 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.089 | 0.392 |
| Number of reflections | 17989 | |
| <I/σ(I)> | 10.4 | 3.3 |
| Completeness [%] | 96.3 | 91.9 |
| Redundancy | 5 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 30% (v/v) PEG 400, 0.1M cacodylate pH 6.5, 0.2M Li2SO4 |
