5WWK
Highly stable green fluorescent protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-12-07 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.100, 95.976, 269.622 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.024 - 3.199 |
| R-factor | 0.2261 |
| Rwork | 0.221 |
| R-free | 0.27470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3v3d |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.963 |
| Data reduction software | XDS (GPLv) |
| Data scaling software | Aimless (0.5.23) |
| Phasing software | PHASER (2.7.16) |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 29.020 | 29.020 | 3.420 |
| High resolution limit [Å] | 3.200 | 9.050 | 3.200 |
| Rmerge | 0.361 | 0.072 | 0.959 |
| Rmeas | 0.375 | 0.075 | 0.996 |
| Rpim | 0.101 | 0.021 | 0.265 |
| Total number of observations | 339416 | 15808 | 62303 |
| Number of reflections | 25406 | ||
| <I/σ(I)> | 8.9 | 25.5 | 3.4 |
| Completeness [%] | 99.8 | 97.4 | 99.7 |
| Redundancy | 13.4 | 12.8 | 13.9 |
| CC(1/2) | 0.983 | 0.998 | 0.844 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | PEG 8000, HEPES pH 8.0, 100 mM Mgcl2 |
