5WWJ

Crystal Structure of HLA-A*2402 in complex with avian influenza A(H7N9) virus-derived peptide H7-25 (data set 1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-10-28
Detector	SDMS
Wavelength(s)	0.97930
Spacegroup name	P 1 21 1
Unit cell lengths	81.315, 67.272, 88.321
Unit cell angles	90.00, 98.41, 90.00

Refinement procedure

Resolution	34.660 - 2.290
R-factor	0.166
Rwork	0.164
R-free	0.21050
RMSD bond length	0.009
RMSD bond angle	1.222
Data reduction software	CNS
Data scaling software	CNS
Phasing software	CNS
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.290
Number of reflections	164558
<I/σ(I)>	41.3
Completeness [%]	99.1
Redundancy	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291	0.1 M Imidazole pH 7.0, 12% w/v Polyethylene glycol 20000