5WWG
Crystal structure of hnRNPA2B1 in complex with AAGGACUUGC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-10-13 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97776 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 132.810, 46.228, 33.994 |
| Unit cell angles | 90.00, 102.22, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.030 |
| R-factor | 0.20145 |
| Rwork | 0.199 |
| R-free | 0.25628 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5en1 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.831 |
| Data reduction software | DENZO |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.082 |
| High resolution limit [Å] | 2.030 | 2.030 |
| Number of reflections | 14087 | |
| <I/σ(I)> | 2.4 | |
| Completeness [%] | 95.9 | |
| Redundancy | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 25% (w/v) PEG 1500, 0.1M MMT/Sodium hydroxide pH 9.0 |
