5WWF
Crystal structure of hnRNPA2B1 in complex with RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-10-13 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97776 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.351, 38.301, 107.931 |
| Unit cell angles | 90.00, 92.43, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.150 |
| R-factor | 0.20549 |
| Rwork | 0.203 |
| R-free | 0.25772 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5en1 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.757 |
| Data reduction software | DENZO |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.230 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Number of reflections | 21583 | |
| <I/σ(I)> | 2.25 | |
| Completeness [%] | 96.8 | |
| Redundancy | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.2 M Lithium sulfate monohydrate, 0.1 M BIS-TRIS pH 6.5, 25% w/v Polyethylene glycol 3350 |
