5WWE
Crystal structure of hnRNPA2B1 in complex with RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-10-13 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97776 |
| Spacegroup name | P 43 |
| Unit cell lengths | 62.591, 62.591, 53.043 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.400 |
| R-factor | 0.2269 |
| Rwork | 0.224 |
| R-free | 0.27434 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5en1 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.581 |
| Data reduction software | DENZO |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 8124 | |
| <I/σ(I)> | 2.333 | |
| Completeness [%] | 100.0 | |
| Redundancy | 13.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 0.1M Sodium citrate/Citric acid pH 5.5, 20% PEG 3000 |
