5WW4
Crystal structure of the second DNA-Binding protein under starvation from Mycobacterium smegmatis soaked with iron in the ratio of 48 iron atoms per dodecamer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-02-25 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54179 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 89.410, 89.410, 420.780 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.000 - 2.050 |
| R-factor | 0.1715 |
| Rwork | 0.169 |
| R-free | 0.21165 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.135 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.480 | 2.140 |
| High resolution limit [Å] | 2.030 | 2.030 |
| Number of reflections | 41426 | |
| <I/σ(I)> | 30.1 | 11.6 |
| Completeness [%] | 97.6 | 83.7 |
| Redundancy | 13.3 | 13.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 6 | 298 | 100 mM sodium cacodylate, 150 mM MgCl2, 20% PEG3000 |
