5WW3
Crystal structure of the second DNA-Binding protein under starvation from Mycobacterium smegmatis soaked with iron in the ratio of 24 iron atoms per dodecamer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2012-02-24 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.54179 |
Spacegroup name | H 3 2 |
Unit cell lengths | 90.170, 90.170, 420.411 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 2.050 |
R-factor | 0.16602 |
Rwork | 0.164 |
R-free | 0.19939 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2z90 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.143 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.610 | 2.140 |
High resolution limit [Å] | 2.030 | 2.030 |
Number of reflections | 41744 | |
<I/σ(I)> | 27.1 | 12.4 |
Completeness [%] | 96.8 | 78.2 |
Redundancy | 8.2 | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 6 | 298 | 100 mM sodium cacodylate, 150 mM MgCl2, 20% PEG3000 |