5WVZ

The crystal structure of Cren7 mutant L28F in complex with dsDNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2010-11-16
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	C 2 2 21
Unit cell lengths	78.791, 79.139, 105.205
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.300
R-factor	0.203
Rwork	0.201
R-free	0.23710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3lwh
RMSD bond length	0.009
RMSD bond angle	1.233
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.042	0.559
Number of reflections	14849
<I/σ(I)>	42.4	2.1
Completeness [%]	99.7	99.3
Redundancy	6.2	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.8	293	30% PEG1500