5WVR

Crystal structure of Osh1 ORD domain in complex with cholesterol

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2016-12-06
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97950
Spacegroup name	P 1 21 1
Unit cell lengths	61.174, 46.602, 73.103
Unit cell angles	90.00, 96.35, 90.00

Refinement procedure

Resolution	22.190 - 2.200
R-factor	0.193
Rwork	0.191
R-free	0.23000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5h2d
RMSD bond length	0.009
RMSD bond angle	1.038
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.240
High resolution limit [Å]	2.200	2.200
Rmerge	0.098	0.373
Number of reflections	20511
<I/σ(I)>	23	5.24
Completeness [%]	97.2	97
Redundancy	3	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	295	Bicine-HCl pH 9.0, 20% PEG 3350, 0.2M MgSO4