5WVF
Crystal structure of a mutant insect group III chitinase (CAD2-E647L) from Ostrinia furnacalis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-06-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 71.475, 71.475, 175.985 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.883 - 2.399 |
| R-factor | 0.2095 |
| Rwork | 0.207 |
| R-free | 0.23280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5wus |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.135 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11_2567: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.440 |
| High resolution limit [Å] | 2.399 | 2.399 |
| Number of reflections | 18731 | |
| <I/σ(I)> | 11.1 | |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 13.5 | 13.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.1 | 277 | 200mM tri-sodium citrate dihydrate, 20% PEG3350 |
