5WUV

Crystal structure of Certolizumab Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2015-04-08
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	58.330, 63.696, 161.415
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.165 - 1.952
R-factor	0.1492
Rwork	0.148
R-free	0.17870
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.060
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	37.960
High resolution limit [Å]	1.950
Number of reflections	43749
<I/σ(I)>	28.2
Completeness [%]	98.2
Redundancy	5.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1M bis-Tris pH5.5, 0.2M Ammunium sulfate, 25% PEG3350