5WUR

Crystal structure of SigW in complex with its anti-sigma RsiW, an oxdized form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2016-10-19
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97926
Spacegroup name	P 21 21 21
Unit cell lengths	63.075, 63.520, 138.547
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.600
R-factor	0.23824
Rwork	0.237
R-free	0.26753
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.311
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.640
High resolution limit [Å]	2.600	2.600
Number of reflections	17578
<I/σ(I)>	46.3	6.4
Completeness [%]	99.1	100
Redundancy	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	7.5	293	PEG3350, isopropanol, calcium chloride