5WUQ

Crystal structure of SigW in complex with its anti-sigma RsiW, a zinc binding form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2016-07-26
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97933
Spacegroup name	P 21 21 21
Unit cell lengths	63.462, 64.206, 138.374
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.800
R-factor	0.21359
Rwork	0.210
R-free	0.28667
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.289
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.850
High resolution limit [Å]	2.800	2.800
Number of reflections	14411
<I/σ(I)>	41.5	5.7
Completeness [%]	98.9	100
Redundancy	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	7.5	293	PEG3350, isopropanol, calcium chloride