5WUN

Crystal structure of mouse importin-alpha1 bound to non-phosphorylated NLS of EBNA1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	95
Detector technology	PIXEL
Collection date	2016-11-13
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	78.510, 90.180, 98.890
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.800 - 2.200
R-factor	0.178
Rwork	0.177
R-free	0.20380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ukw
RMSD bond length	0.004
RMSD bond angle	0.683
Data reduction software	MOSFLM
Data scaling software	Aimless (0.5.28)
Phasing software	MOLREP
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	28.800	28.800	2.270
High resolution limit [Å]	2.200	9.070	2.200
Rmerge	0.087	0.021	0.990
Rmeas	0.095
Rpim	0.038
Total number of observations	218370
Number of reflections	36317
<I/σ(I)>	14.1
Completeness [%]	99.9	97.1	99.4
Redundancy	6	5.2	5.2
CC(1/2)	0.998	0.995	0.599

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	MES, sodium citrate, DTT