5WUJ

Crystal structure of FliF-FliG complex from H. pylori

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-07-06
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.98
Spacegroup name	P 32 2 1
Unit cell lengths	61.098, 61.098, 86.930
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.198 - 2.300
R-factor	0.1902
Rwork	0.188
R-free	0.22370
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	0.810
Data reduction software	MOSFLM
Data scaling software	SCALA (0.1.27)
Phasing software	AutoSol
Refinement software	PHENIX ((dev_2621: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.200	45.200	2.390
High resolution limit [Å]	2.300	8.600	2.300
Rmerge	0.121	0.101	0.336
Number of reflections	8475
<I/σ(I)>	17
Completeness [%]	96.6	95.8	99.9
Redundancy	20.1	11	20.9
CC(1/2)	0.994	0.973	0.992

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	289	0.1 M Sodium citrate tribasic dihydrate, 20%(v/v) 2-Propanol, 20%(w/v) Polyethylene glycol 4000