5WU5

Crystal structure of apo human Tut1, form III

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	95
Detector technology	PIXEL
Collection date	2015-06-25
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.98
Spacegroup name	P 43 21 2
Unit cell lengths	173.140, 173.140, 208.580
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.989 - 3.404
R-factor	0.2162
Rwork	0.214
R-free	0.25820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5wu2
RMSD bond length	0.003
RMSD bond angle	0.839
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000		3.490
High resolution limit [Å]	3.400	15.220	3.400
Rmerge	0.334	0.059	1.281
Number of reflections	39562
<I/σ(I)>	8.7	28.29	2.32
Completeness [%]	89.8	95.7	32.1
Redundancy	13
CC(1/2)	0.993	0.998	0.711

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	Sodium Cacodylate pH 7.0, PEG4000, Tacsimate