5WU4

Crystal structure of human Tut1 bound with MgATP, form II

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	95
Detector technology	PIXEL
Collection date	2015-06-15
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.98
Spacegroup name	P 1 21 1
Unit cell lengths	77.660, 88.560, 93.820
Unit cell angles	90.00, 98.66, 90.00

Refinement procedure

Resolution	19.980 - 2.800
R-factor	0.2431
Rwork	0.241
R-free	0.28240
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5wu2
RMSD bond length	0.003
RMSD bond angle	0.890
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000		2.870
High resolution limit [Å]	2.800	12.520	2.800
Rmerge	0.134	0.031	1.527
Number of reflections	31113
<I/σ(I)>	13.14	44.93	1.3
Completeness [%]	99.7	97.3	98.5
Redundancy	6.8
CC(1/2)	0.998	0.999	0.461

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		297	Hepes pH 7.5, PEG3350, Tacsimate