5WTX

Structure of Nop9

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2013-12-28
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9793
Spacegroup name	I 21 21 21
Unit cell lengths	93.801, 134.846, 145.921
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.517 - 3.074
R-factor	0.1977
Rwork	0.194
R-free	0.26590
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.275
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.074
Number of reflections	17549
<I/σ(I)>	17.1
Completeness [%]	99.7
Redundancy	14.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	sodium sulfate decahydrate, PEG 3350