5WTR

Crystal structure of a prokaryotic TRIC channel in 0.5 M KCl

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2012-04-08
Detector	ADSC QUANTUM 315
Wavelength(s)	1.00137
Spacegroup name	C 1 2 1
Unit cell lengths	152.073, 87.730, 173.062
Unit cell angles	90.00, 108.90, 90.00

Refinement procedure

Resolution	50.000 - 2.200
Rwork	0.211
R-free	0.22850
Structure solution method	SAD
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	AutoSol
Refinement software	CNS (1.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.320
High resolution limit [Å]	2.200	2.200
Rmerge		0.604
Number of reflections	106700
<I/σ(I)>	7.1	2.7
Completeness [%]	97.4	94.8
Redundancy	4.7	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	289	Well solution: 22-26% PEG 3000, 0.1 M Tris-HCl pH8.0, 0.2 M NaAc, 0.2 M KCl Before being frozen, the crystals were washed and soaked in 26% PEG3000, 50 mM Tris-HCl pH 8.0, 1.1% OG, 0.1% DM, 3% ethylene glycol, 0.5 M KCl