5WTR
Crystal structure of a prokaryotic TRIC channel in 0.5 M KCl
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-04-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.00137 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 152.073, 87.730, 173.062 |
| Unit cell angles | 90.00, 108.90, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.200 |
| Rwork | 0.211 |
| R-free | 0.22850 |
| Structure solution method | SAD |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | AutoSol |
| Refinement software | CNS (1.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.604 | |
| Number of reflections | 106700 | |
| <I/σ(I)> | 7.1 | 2.7 |
| Completeness [%] | 97.4 | 94.8 |
| Redundancy | 4.7 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 289 | Well solution: 22-26% PEG 3000, 0.1 M Tris-HCl pH8.0, 0.2 M NaAc, 0.2 M KCl Before being frozen, the crystals were washed and soaked in 26% PEG3000, 50 mM Tris-HCl pH 8.0, 1.1% OG, 0.1% DM, 3% ethylene glycol, 0.5 M KCl |
