5WTK

Crystal structure of RNP complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2016-09-16
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9789
Spacegroup name	P 21 21 21
Unit cell lengths	86.589, 137.051, 153.877
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.405 - 2.651
R-factor	0.2594
Rwork	0.259
R-free	0.27160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5wtj
RMSD bond length	0.012
RMSD bond angle	1.584
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.650
Rmerge		0.625
Number of reflections	53055
<I/σ(I)>	13.4
Completeness [%]	98.5
Redundancy	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.1M Sodium malonate, 12% PEG 3350