5WTI
Crystal structure of the CRISPR-associated protein in complex with crRNA and DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-10-29 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.9785 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 172.056, 138.742, 95.611 |
| Unit cell angles | 90.00, 117.45, 90.00 |
Refinement procedure
| Resolution | 19.910 - 2.682 |
| R-factor | 0.2185 |
| Rwork | 0.217 |
| R-free | 0.26440 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.717 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.600 |
| Number of reflections | 86779 |
| <I/σ(I)> | 23.3 |
| Completeness [%] | 99.0 |
| Redundancy | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 289 | 0.08 M Strontium chloride hexahydrate, 0.04 M Sodium cacodylate trihydrate, 35% v/v (+/-)-2-Methyl-2,4-pentanediol, 0.012 M Spermine tetrahydrochloride |
