5WTG
Crystal structure of the Fab fragment of anti-HAV antibody R10
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-02 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.997 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.512, 140.522, 68.924 |
| Unit cell angles | 90.00, 110.36, 90.00 |
Refinement procedure
| Resolution | 49.232 - 2.907 |
| R-factor | 0.2042 |
| Rwork | 0.201 |
| R-free | 0.26640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ejo |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.175 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.200 | |
| Number of reflections | 20094 | |
| <I/σ(I)> | 3.68 | |
| Completeness [%] | 97.9 | 95.6 |
| Redundancy | 3.1 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 20% PEG3350, 0.2M Sodium Thiocyanate |
