5WTA
Crystal Structure of Staphylococcus aureus SdrE apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-06-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 1 |
| Unit cell lengths | 41.810, 61.730, 139.610 |
| Unit cell angles | 80.89, 89.83, 73.34 |
Refinement procedure
| Resolution | 38.930 - 2.300 |
| R-factor | 0.19693 |
| Rwork | 0.194 |
| R-free | 0.25171 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1r17 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.409 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.010 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 55992 | 8137 |
| <I/σ(I)> | 7.8 | 3.4 |
| Completeness [%] | 95.5 | 95.2 |
| Redundancy | 2.2 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.1M Citric acid, pH 3.5, 16% PEG 3350 (w/v), 2% MPD (v/v) |
