5WSZ

Crystal structure of a lytic polysaccharide monooxygenase,BtLPMO10A, from Bacillus thuringiensis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-10-17
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.97776
Spacegroup name	P 21 21 21
Unit cell lengths	73.021, 73.446, 113.583
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.118 - 2.565
R-factor	0.2058
Rwork	0.203
R-free	0.25040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2bem
RMSD bond length	0.003
RMSD bond angle	0.841
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.660
High resolution limit [Å]	2.570	2.570
Rmerge	0.195	0.784
Rpim	0.072	0.310
Number of reflections	20252
<I/σ(I)>	10.9
Completeness [%]	100.0	91.06
Redundancy	7.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.2M magnesium acetate tetrahydrate, 0.1M sodium cacodylate trihydrate pH 6.5, 20% w/v Polyethylene glycol 8000