5WSU
Crystal structure of Myosin VIIa IQ5-SAH in complex with apo-CaM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0300 |
| Spacegroup name | P 41 |
| Unit cell lengths | 80.930, 80.930, 175.290 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.917 - 3.000 |
| R-factor | 0.2308 |
| Rwork | 0.224 |
| R-free | 0.29010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ix7 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.937 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.131 | 0.967 |
| Number of reflections | 22489 | |
| <I/σ(I)> | 9.3 | 2 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 6.7 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.6 | 289 | 0.1M sodium citrate tribasic (pH 5.6), 20%(v/v) isopropanol, 20%(w/v) PEG 4,000 |
