5WRW

Structure of human apo-SRP72

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2015-06-20
Detector	RIGAKU
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	83.435, 123.715, 150.723
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.010 - 2.910
R-factor	0.23371
Rwork	0.232
R-free	0.26640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5wrv
RMSD bond length	0.009
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.910
Number of reflections	35059
<I/σ(I)>	26.2
Completeness [%]	100.0
Redundancy	7.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	(NH4)2SO4