5WRR

Crystal structure of Fam20A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2016-01-14
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 32 2 1
Unit cell lengths	156.854, 156.854, 143.655
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.136 - 2.506
R-factor	0.2158
Rwork	0.215
R-free	0.23140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4kqa
RMSD bond length	0.002
RMSD bond angle	0.617
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.540
High resolution limit [Å]	2.500	6.780	2.500
Rmerge	0.172	0.088	1.804
Number of reflections	70256
<I/σ(I)>	9.4
Completeness [%]	99.9	98.5	100
Redundancy	10.9	9.7	11.1
CC(1/2)		0.993	0.841

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	291	ammonium sulfate, HEPES, PEG 400