5WRB
Crystal structure of hen egg-white lysozyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | FREE ELECTRON LASER |
| Source details | SACLA BEAMLINE BL3 |
| Synchrotron site | SACLA |
| Beamline | BL3 |
| Temperature [K] | 299 |
| Detector technology | CCD |
| Collection date | 2015-11-22 |
| Detector | MPCCD |
| Wavelength(s) | 1.77 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 79.600, 79.600, 38.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.143 - 2.013 |
| R-factor | 0.181 |
| Rwork | 0.180 |
| R-free | 0.20180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.070 |
| Data scaling software | CrystFEL |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.000 |
| Number of reflections | 8750 |
| <I/σ(I)> | 10.5 |
| Completeness [%] | 100.0 |
| Redundancy | 393 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 290 | sodium chloride, PEG 6000, pH 3.0 |
