5WQX
Covalent bond formation of synthetic ligand with hPPARg-LBD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-05-28 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 92.910, 61.200, 118.020 |
| Unit cell angles | 90.00, 102.82, 90.00 |
Refinement procedure
| Resolution | 50.710 - 2.290 |
| R-factor | 0.23182 |
| Rwork | 0.229 |
| R-free | 0.27711 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.574 |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.710 |
| High resolution limit [Å] | 2.290 |
| Number of reflections | 21980 |
| <I/σ(I)> | 10.5 |
| Completeness [%] | 74.8 |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | 0.1M Tris-HCl PH 7.4, 0.8M sodium citrate |
