5WQ0

Receiver domain of Spo0A from Paenisporosarcina sp. TG-14

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2015-10-14
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97933
Spacegroup name	P 21 21 21
Unit cell lengths	56.969, 72.233, 189.650
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.572 - 2.604
R-factor	0.1898
Rwork	0.187
R-free	0.24260
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.344
Data scaling software	HKL-2000
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.600
Number of reflections	24741
<I/σ(I)>	11.1
Completeness [%]	98.3
Redundancy	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2M magnesium chloride, 26%(w/v) PEG3350, 0.1M HEPES pH 7.5