5WPU
Crystal structure HpiC1 Y101S
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-07 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.03 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 113.936, 49.622, 53.438 |
| Unit cell angles | 90.00, 110.31, 90.00 |
Refinement procedure
| Resolution | 45.213 - 1.390 |
| R-factor | 0.1512 |
| Rwork | 0.149 |
| R-free | 0.18170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5wpp |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.794 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 45.213 |
| High resolution limit [Å] | 1.390 |
| Number of reflections | 52971 |
| <I/σ(I)> | 18.24 |
| Completeness [%] | 93.6 |
| Redundancy | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 20% MEPEG 5000, 150 mM CaCl2, 100 mM Tris pH 8.5, 5% ethylene glycol |
