5WP5
Arabidopsis thaliana phosphoethanolamine N-methyltransferase 2 (AtPMT2) in complex with SAH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2011-07-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.055, 90.224, 122.875 |
| Unit cell angles | 90.00, 100.14, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.500 |
| R-factor | 0.1609 |
| Rwork | 0.160 |
| R-free | 0.17890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB entries 4KRG & 4KRH |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.071 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 162824 | |
| <I/σ(I)> | 37.4 | |
| Completeness [%] | 99.1 | |
| Redundancy | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 200 mM potassium formate, 20% w/v PEG3350 |
