5WNV

Crystal Structure of 30S ribosomal subunit from Thermus thermophilus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.98
Spacegroup name	P 41 21 2
Unit cell lengths	400.000, 400.000, 173.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.801 - 3.300
R-factor	0.1791
Rwork	0.179
R-free	0.20250
RMSD bond length	0.002
RMSD bond angle	0.502
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	39.801	39.801	2.990
High resolution limit [Å]	2.920	13.050	2.920
Rmerge	0.570	0.039
Rmeas	0.573	0.039
Total number of observations	31531631
Number of reflections	296028	3516	17704
<I/σ(I)>	14.44	122.24
Completeness [%]	98.3	94.4	80.1
Redundancy	106.516	90.252	70.208
CC(1/2)	1.000	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	6.5	298	17% MPD