5WNT

Crystal Structure of 30S ribosomal subunit from Thermus thermophilus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.98
Spacegroup name	P 41 21 2
Unit cell lengths	400.000, 400.000, 173.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.779 - 3.300
R-factor	0.1734
Rwork	0.173
R-free	0.21170
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.002
RMSD bond angle	0.534
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	39.779	39.779	2.990
High resolution limit [Å]	2.910	13.030	2.910
Rmerge	0.525	0.048
Rmeas	0.528	0.048
Total number of observations	30978832
Number of reflections	290551	3474	13804
<I/σ(I)>	14.94	94.79	0.01
Completeness [%]	96.1	93	62.2
Redundancy	106.621	87.421	81.45
CC(1/2)	1.000	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	6.5	298	17% MPD