5WNP

Crystal Structure of 30S ribosomal subunit from Thermus thermophilus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.98
Spacegroup name	P 41 21 2
Unit cell lengths	400.000, 400.000, 173.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.981 - 3.300
R-factor	0.1917
Rwork	0.191
R-free	0.22430
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.002
RMSD bond angle	0.503
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.981	29.981	2.980
High resolution limit [Å]	2.900	12.980	2.900
Rmerge	0.830	0.068
Rmeas	0.835	0.069
Total number of observations	23797436
Number of reflections	298139	3576	15653
<I/σ(I)>	7.29	58.08
Completeness [%]	97.6	94.9	69.8
Redundancy	79.82	66.716	35.337
CC(1/2)	0.999	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	6.5	298	17% MPD