5WN9

Structure of antibody 2D10 bound to the central conserved region of RSV G

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-11
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.033
Spacegroup name	P 21 21 21
Unit cell lengths	44.843, 56.387, 126.148
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.253 - 1.551
R-factor	0.1696
Rwork	0.169
R-free	0.18510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ncj 5k9q
RMSD bond length	0.008
RMSD bond angle	0.968
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	42.253	50.000	1.580
High resolution limit [Å]	1.550	4.210	1.550
Rmerge	0.070	0.047	0.953
Rmeas	0.074	0.050
Rpim	0.023	0.016	0.389
Number of reflections	47198	2587	2290
<I/σ(I)>	8.2
Completeness [%]	99.9	99.8	99.4
Redundancy	9.6	9.7	6.2
CC(1/2)		0.999	0.751

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	295.15	25% PEG4000, 0.17M Ammonium acetate, 0.085 sodium citrate pH 5.6