5WMI
Arabidopsis thaliana Prephenate Aminotransferase mutant- T84V
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2015-12-02 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 117.038, 67.721, 51.557 |
| Unit cell angles | 90.00, 103.77, 90.00 |
Refinement procedure
| Resolution | 30.680 - 2.000 |
| R-factor | 0.1691 |
| Rwork | 0.167 |
| R-free | 0.21510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5wmh |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.025 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.680 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.075 | 0.253 |
| Number of reflections | 25739 | 1336 |
| <I/σ(I)> | 32.35 | 5.56 |
| Completeness [%] | 96.8 | 100 |
| Redundancy | 3.6 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 277 | 20% (w/v) PEG-6000, 100 mM citric acid, pH 5.0 |
