5WME
Crystal Structure of Amino Acids 1729-1786 of Human Beta Cardiac Myosin Fused to Gp7 as Anti-Parallel Four-Helix Bundle
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-12-13 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97924 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 24.247, 72.960, 114.484 |
| Unit cell angles | 90.00, 96.29, 90.00 |
Refinement procedure
| Resolution | 44.867 - 2.300 |
| R-factor | 0.2262 |
| Rwork | 0.224 |
| R-free | 0.26430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1no4 |
| RMSD bond length | 0.001 |
| RMSD bond angle | 0.325 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.075 | 0.396 |
| Number of reflections | 17830 | 868 |
| <I/σ(I)> | 18.1 | 4.7 |
| Completeness [%] | 99.4 | 97 |
| Redundancy | 4.1 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | 18% (w/v) pentaerythritol ethoxylate 797, 150 mM ammonium thiocyanate, 100 mM sodium acetate pH 5.0. |
