5WMB
Structure of the 10S (-)-cis-BP-dG modified Rev1 ternary complex (the BP residue is disordered)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-10-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.10 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.307, 65.476, 131.657 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 65.830 - 2.250 |
| R-factor | 0.17223 |
| Rwork | 0.170 |
| R-free | 0.21710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2aq4 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.474 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 65.830 |
| High resolution limit [Å] | 2.250 |
| Number of reflections | 26824 |
| <I/σ(I)> | 18.6 |
| Completeness [%] | 100.0 |
| Redundancy | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 0.25 M sodium citrate pH 6.0 buffer 15-20% PEG3350 |
