5WLS

Crystal Structure of a Pollen Receptor Kinase 3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	77
Detector technology	CCD
Collection date	2017-06-13
Detector	RAYONIX MX300-HS
Wavelength(s)	1.0422
Spacegroup name	P 42
Unit cell lengths	72.061, 72.061, 125.677
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.220 - 2.496
R-factor	0.248
Rwork	0.246
R-free	0.27660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4lsx
RMSD bond length	0.006
RMSD bond angle	1.316
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	36.220
High resolution limit [Å]	2.496
Number of reflections	22282
<I/σ(I)>	24.52
Completeness [%]	99.8
Redundancy	7.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294.15	0.1M Tris, pH 8.0, 18% PEG3350