5WL1
Crystal Structure of Human CD1b in Complex with PG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-06-27 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.9573 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.889, 80.280, 92.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.340 - 1.380 |
| R-factor | 0.189 |
| Rwork | 0.188 |
| R-free | 0.20600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.090 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.990 | 1.400 |
| High resolution limit [Å] | 1.380 | 1.380 |
| Rpim | 0.056 | |
| Number of reflections | 87712 | 4196 |
| <I/σ(I)> | 17.1 | 2 |
| Completeness [%] | 98.4 | 96.8 |
| Redundancy | 14.7 | 14.8 |
| CC(1/2) | 0.623 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 26% PEG 3350, 0.2M Sodium Malonate |
