5WKV

Structure of an acid sensing ion channel in a resting state with calcium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-08-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97870
Spacegroup name	P 21 21 21
Unit cell lengths	109.180, 133.700, 157.650
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.951 - 3.200
R-factor	0.2875
Rwork	0.287
R-free	0.29710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2qts
RMSD bond length	0.003
RMSD bond angle	0.688
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((dev_2597: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	100.000
High resolution limit [Å]	3.200
Number of reflections	37779
<I/σ(I)>	7.05
Completeness [%]	97.5
Redundancy	5.76
CC(1/2)	0.979

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	277	100 mM Tris pH 8.5, 150 mM NaCl, 19% PEG 1000, 5 mM CaCl2.