5WKU

Structure of an acid sensing ion channel in a resting state with barium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-05-07
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.99
Spacegroup name	P 21 21 21
Unit cell lengths	109.939, 130.408, 157.850
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.881 - 2.950
R-factor	0.2278
Rwork	0.226
R-free	0.25820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2qts
RMSD bond length	0.004
RMSD bond angle	0.701
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((dev_2597: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.950
Number of reflections	48478
<I/σ(I)>	15.39
Completeness [%]	100.0
Redundancy	20.6
CC(1/2)	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	277	100 mM Tris pH 8.5, 28% PEG 400, 150 mM NaCl, 5 mM BaCl2.