5WKA
Crystal structure of a GH1 beta-glucosidase retrieved from microbial metagenome of Poraque Amazon lake
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE W01B-MX2 |
| Synchrotron site | LNLS |
| Beamline | W01B-MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-09-27 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.459 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 165.471, 56.652, 209.546 |
| Unit cell angles | 90.00, 96.66, 90.00 |
Refinement procedure
| Resolution | 46.463 - 2.750 |
| R-factor | 0.2339 |
| Rwork | 0.232 |
| R-free | 0.27030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gnx |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.672 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 46.463 |
| High resolution limit [Å] | 2.750 |
| Number of reflections | 49452 |
| <I/σ(I)> | 6 |
| Completeness [%] | 88.2 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1 M MES pH 6.0, PEG 6000 20% (w/v) and 0.2 M sodium chloride. |
