5WK9
R186AP450cam with CN and camphor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL14-1 |
| Synchrotron site | SSRL |
| Beamline | BL14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-07-28 |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.950, 58.860, 57.280 |
| Unit cell angles | 90.00, 104.99, 90.00 |
Refinement procedure
| Resolution | 45.309 - 1.983 |
| R-factor | 0.1673 |
| Rwork | 0.163 |
| R-free | 0.21540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cpp |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.196 |
| Data reduction software | iMOSFLM |
| Data scaling software | pointless |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.000 | |
| High resolution limit [Å] | 1.980 | 1.980 |
| Rpim | 0.049 | 0.202 |
| Number of reflections | 25408 | 1773 |
| <I/σ(I)> | 8.1 | 2.8 |
| Completeness [%] | 99.5 | 98.5 |
| Redundancy | 3.7 | 3.6 |
| CC(1/2) | 0.996 | 0.921 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 298 | 50 mM potassium phosphate pH 7.4, 400 mM KCl, 32%PEG4000 |
