5WK4

Crystal structure of an anti-idiotype VLR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 1
Unit cell lengths	60.415, 61.382, 80.562
Unit cell angles	110.09, 99.79, 93.52

Refinement procedure

Resolution	34.720 - 1.498
R-factor	0.1949
Rwork	0.193
R-free	0.22340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3twi modified
RMSD bond length	0.006
RMSD bond angle	0.871
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.720	1.552
High resolution limit [Å]	1.498	1.498
Rmerge	0.064	0.736
Rmeas	0.075	0.859
Rpim	0.039	0.441
Number of reflections	145338	9013
<I/σ(I)>	11.05	1.49
Completeness [%]	85.1	52.73
Redundancy	3.7	3.7
CC(1/2)	0.997	0.732

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	277	0.1M Tris pH8.5 0.2M magnesium chloride 24%(w/v) peg-4000