5WK4
Crystal structure of an anti-idiotype VLR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-19 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 1 |
| Unit cell lengths | 60.415, 61.382, 80.562 |
| Unit cell angles | 110.09, 99.79, 93.52 |
Refinement procedure
| Resolution | 34.720 - 1.498 |
| R-factor | 0.1949 |
| Rwork | 0.193 |
| R-free | 0.22340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3twi modified |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.871 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.720 | 1.552 |
| High resolution limit [Å] | 1.498 | 1.498 |
| Rmerge | 0.064 | 0.736 |
| Rmeas | 0.075 | 0.859 |
| Rpim | 0.039 | 0.441 |
| Number of reflections | 145338 | 9013 |
| <I/σ(I)> | 11.05 | 1.49 |
| Completeness [%] | 85.1 | 52.73 |
| Redundancy | 3.7 | 3.7 |
| CC(1/2) | 0.997 | 0.732 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 0.1M Tris pH8.5 0.2M magnesium chloride 24%(w/v) peg-4000 |
